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Loose Connective Tissue

As the name implies, loose connective tissue consists of a loosely woven mix of fibers, cells, and ground substance.  Areolar, a more technical name used for this tissue type means "spaces".  Loose connective tissue therefore possesses randomly arranged protein fibers with abundant intercellular spaces.  Scattered within the spaces are 7 cell types worth mentioning for their physiological roles:

  1. fibroblasts-elongate or tapered, flattened cells that form the protein fibers and ground substance.  Fibroblasts can often be seen adhering to newly formed fibers.
  2. macrophages or histiocytes-amoeboid or fixed phagocytic cells derived from monocytes of the blood.  Macrophages play a role in phagocytosis(non-specific body defense) and thereafter enter the lymphatic system to aid in activation of immune responses(specific body defense) as antigen presenting cells or APC's.
  3. leukocytes-these blood borne defensive cells leave the vascular system during inflammatory events to patrol adjoining tissue spaces.  Most are neutrophils or lymphocytes.
  4. plasma cells-are lymphatic cells derived from B-lymphocytes.  Plasma cells are highly efficient producers of antibodies.  Although most are located in lymphatic tissues like lymph nodes, they do occur in loose c.t. areas.  Plasma cells, like lymphocytes, possess a well-defined, spherical shape with rounded nuclei .
  5. mast cells-This is the key cell involved in inflammation!  Mast cells release histamine and heparin.  These chemical agents induce the vascular responses that cause inflammation.  Mast cells are fragile, granular cells that can rupture during tissue preparation and staining.
  6. adipocytes-are the cells involved in the metabolism and storage of fats or triglycerides.  During tissue preparation for staining fats are leached from these cells by alcohols.  In most stained slides, they appear as spherical, empty cells with nuclei pushed to the periphery. 
  7. mesenchymal cells-these are the embryonic stem cells that divide to form new cells of all connective tissue types.  Think of the mesenchyme cell as the precursor to fibroblasts, chondroblasts, and osteoblasts.  

Areolar connective tissue is abundant and typically found in most body locales.  As the underlying connective tissue to most epithelia it is a key component of membranes.  Areolar connective tissue also forms much of the mesentery supporting organs within the abdominal cavity.  Also, as a supportive material for blood vessels and nerves, areolar connective tissue is found in the nooks and crannies of most tissues where blood vessels and nerves penetrate.

Since loose connective tissue is so abundant there is variety in its appearance.  Remember, open spaces are typical of this tissue type.  Protein fibers are randomly arranged and are smaller than the fibers of dense irregular connective tissues.  In addition, where you find adipose, there will likely be loose c.t. nearby.

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