MicroBiology Home   Microbiology, 4/e               Prescott, Harley, Klein

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Chapter 14
Supplemental Quiz Question

A new weight loss genetic engineering lab has just created a new strain of E. coli. Hoping to capture the market with a new and better weight-loss product, the new strain is engineered with a plasmid containing genes for enzymes that digest carbohydrates.

1. You obtain a specimen and want to isolate the new strain of E. coli. The laboratory conditions which will best allow you to separate the new strain of E. coli from the wildtype strain of E. coli are?
a. nutrient agar, pH 7.0, and 0% oxygen
b. cellulose agar, pH 3.0, and 20% oxygen
c. nutrient agar, pH 3.0, and 20% oxygen
d. cellulose agar, pH 3.0, and 0% oxygen
e. cellulose agar, pH 7.0, and 20% oxygen

2. After 24 hours, the entire broth culture contains bacteria that demonstrate the phenotype of the engineered strain. What is the likely mechanism for this?
a. Transduction
b. Translation
c. Conjugation
d. Transformation


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