Molecular Biology 2nd Edition               Robert F. Weaver
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MOLECULAR BIOLOGY, second edition Robert F. Weaver, University of Kansas (ISBN: 007-234517-9) pub date July of 2001 NEW to this edition: Completely new chapter (24) on Genomics New topics on DNA damage and repair in a significantly reworked Part VII (Chapters 20-23). See detailed table of contents for specifics. Updated information on numerous topics Revised and new art work throughout Primary headings numbered for easy reference All line art and tables, and many photographs will be available as jpeg files on the VRL and website (See new detailed table of contents for more information) SUMMARY OF CHANGES IN THE SECOND EDITION: PART I INTRODUCTION A Brief History The Molecular Nature of Genes updated table on the forms of DNA updated table on sizes of various DNA An Introduction to Gene Function new discussion of translation elongation, termination of translation and mRNA structure including two new illustrations - Summary of translation elongation, and Simplified gene and mRNA structure. PART II METHODS IN MOLECULAR BIOLOGY Molecular Cloning Methods new and significantly update information on cDNA Cloning, Rapid Amplification of cDNA Ends (RACE), Expression Vectors that Produce Fusion Proteins Molecular Tools for Studying Genes and Gene Activity significantly revised sections on Molecular Separations, and S1 Mapping; new sections on Two-dimensional Gel Electrophoresis, Ion Exchange Chromatography, Gel Filtration Chromatography, including new illustrations covering the Principles of gel filtration chromatography, Two-dimensional gel electrophoresis, SDS-polyacrylamide gel electrophoresis (SDS-PAGE), and ion exchange chromatography. PART III TRANSCRIPTION IN PROKARYOTES The Transcription Apparatus of Prokaryotes new discussion of the experiments of Gamper and Hearst in which they estimated the number of base pairs melted by polymerases in actively-transcribing complexes. New discussion of protein folding includes coverage of limited proteolysis analysis as used by Gourse and Ebright. Significantly revised section on The Roles of b' and b in DNA Binding. New section on the Role of a in Polymerase Assembly, Structure of the Elongation Complex, and The RNA-DNA Hybrid. Operons: Fine Control of Prokaryotic Transcription chapter 9 material on lac operators moved here to join new discussion of the experiments of Thomas Record, et al on the mechanism of repression and the experiments of Benno Muller-Hill and Mitchell Lewis. New illustrations include Effect of lac repressor on access of RNA polymerase to the lac promoter Major Shifts in Prokaryotic Transcription updates DNA-Protein Interactions in Prokaryotes significantly revised section on The Lambda Family of Repressors PART IV TRANSCRIPTION IN EUKARYOTES Eukaryotic RNA Polymerases and Their Promoters significantly revised section on The Roles of the Three RNA Polymerases including new table on RNA Polymerase Specificities. New section on The Shape of RNA Polymerase features the break-through in 1999 of Roger Kornberg and his colleagues in the use of mutant yeast polymerase to facilitate the production of crystals of RNA polymerase. New illustrations include A General Model for Finding a Gene Mutagenized with a P Element, Model of Yeast RNA Polymerase II, Structure of a Yeast RNA Polymerase II-Nucleic Acid Complex, General Transcription Factors in Eukaryotes reorganized topics for more logical flow of information; new section on the work of Green and Young with genome-wide analysis of the effects of temperature-sensitive mutations in yeast TAFII145; new section covering IIS Stimulation of Proofreading Transcripts. New illustrations include The Drosophila tudor control region, Three-dimensional models of TFIID and TFTC, The Preinitiation Complex Envisioned by Tjian and Reinberg, A Model for Proofreading by RNA Polymerase I Transcription Activators in Eukaryotes revised coverage of DNA-binding Domains, revised coverage of Homeodomains, new section covering Recruitment of TFIID, new coverage of Insulators, new section covering the Regulation of Transcription Factors. Numerous new illustrations. Chromatin Structure and its Effects on Transcription new discussion of the architecture of histones, new section covering Chromatin Remodeling. New illustrations include A Model of Telomere Structure and Chromatin Immunoprecipitation. PART V POST-TRANSCRIPTIONAL EVENTS Post-Transcriptional Events I: Splicing new discussion of 3' splice site selection, general updates Post-Transcriptional Events II: Capping and Polyadenylation revised discussion of Basic Mechanism of Polyadenylation, new discussion of Cleavage and Polyadenylation of a Pre-mRNA Post-Transcriptional Events III: Other Events simplified coverage overall, including new illustrations PART VI TRANSLATION The Mechanism of Translation I: Initiation updates and corrections, new discussion of Function of eIF1 and eIF1A, new illustrations include Principle of toeprint assay, Results of toeprint assay, and Effect of competitor RNA on formation of 48S complex. The Mechanism of Translation II: Elongation and Termination many updates include significant revision of discussion on deviations from the universal genetic code, expanded explanations on how EF-Ts perform its exchange function, and on the delicate balance between speed and accuracy of translation. Ribosomes and Transfer RNA reorganized chapter content features new discussion of the Fine Structure of the 70S ribosome examines the1999 work of Harry Noller; significantly revised discussion of the Fine Structure of the 30S Subunit includes the 2000 work of V Ramakrishnan that revealed the crystal structure of the Thermus thermophilus 30S subunit. New discussion of the fine structure of the 50S subunit features the 2000 x-ray crystallography work of Peter Moore and Thomas Steitz showing that rRNA, not protein, is at the active center of peptidyl transferase. Numerous new illustrations. PART VII DNA REPLICATION, RECOMBINATION, AND TRANSPOSITION DNA Replication I: Basic Mechanism and Enzymology completely new section on DNA damage and repair DNA Replication II: Detailed Mechanism significantly revised coverage of Initiation includes new discussion of Priming in E. coli and The Origin of Replication in E. coli and restructured coverage of Priming in Eukaryotes. Homologous Recombination chapter now focuses exclusively on homologous recombination; new section on Meiotic Recombination covers overview of the process, the double-stranded DNA break, and the creation of single-stranded ends at DSBs. Among new illustrations are models of RUV protein-junction complexes, a model for meiotic recombination in yeast, a model for gene conversion in sporulating Neurospora, and a model for gene conversion without mismatch repair. Site-Specific Recombination and Transposition chapter begins with the topics from the second half of chapter 22 in the first edition with considerable updates, and an entirely new discussion of Retrotransposons that includes coverage of Retroviruses, Retrotransposons, Non-LRT Retrotransposons, Non-Authonomous Retrotranposons, and Group II Introns. Numerous new illustrations. Part VIII Genomes Genomics entirely new chapter begins with a discussion of the first sequenced genomes, then moves on to topics like the Human Genome Project, Vectors for Large-Scale Genome Projects, The Clone-by-Clone Strategy, Shotgun Sequencing, Sequencing Standards, and a major section on Applications of Genomics (review detailed table of contents).

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