Chapter 14
- Termination by RNA polymerase II is dependent on a functional polyadenylation signal (see Chapter 15, below). Dye and Proudfoot ( show that termination also depends on a functional downstream splice site in the last intron, at least in the human - and -globin genes. Thus, termination appears to be linked to both splicing and polyadenylation. Dye, M.J. and N.J. Proudfoot. 1999. Terminal exon definition occurs cotranscriptionally and promotes termination of RNA polymerase II. Molecular Cell 3:371-78)
- Stark, et al. have used single-molecule electron cryomicroscopy to determine the three-dimensional structure of human U1 snRNP to 10 resolution. They found a 70-80 donut-shaped central element containing the seven Sm proteins found in all snRNPs. Taking into account other published data, these studies allow all the proteins and RNA in U1 snRNP to be placed in a model. (Stark, H., P. Dube, R. Lührmann, and B. Kastner. 2001. Arrangement of RNA and proteins in the spliceosomal U1 small nuclear ribonucleoprotein particle. Nature 409:539-42)
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