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Molecular Biology 2nd Edition Robert F. Weaver | ||||||
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Chapter 15
- Termination of transcription by RNA polymerase II occurs after cleavage at the polyadenylation site, and hundreds or thousands of bp downstream of that site. Dye and Proudfoot show that two distinct steps are involved. First, the polymerase must traverse a pretermination cleavage (PTC) sequence that leads to cleavage of the elongating transcript downstream of the polyadenylation site. Second, the polymerase is released from the template DNA. The first step is not dependent on a functional polyadenylation signal, but the second is. (Dye, M.J. and N.J. Proudfoot. 2001. Multiple transcript cleavage precedes polymerase release in termination by RNA polymerase II. Cell 105:669-81)
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