Table of Contents

PART I INTRODUCTION

1. A Brief History
2. The Molecular Nature of Genes
3. An Introduction to Gene Function

PART II METHODS IN MOLECULAR BIOLOGY

4. Molecular Cloning Methods
5. Molecular Tools for Studying Genes and Gene Activity

PART III TRANSCRIPTION IN PROKARYOTES

6. The Transcription Apparatus of Prokaryotes
7. Operons: Fine Control of Prokaryotic Transcription
8. Major Shifts in Prokaryotic Transcription
9. DNA-Protein Interactions in Prokaryotes

PART IV TRANSCRIPTION IN EUKARYOTES

10. Eukaryotic RNA Polymerases and Their Promoters
11. General Transcription Factors in Eukaryotes
12. Transcription Activators in Eukaryotes
13. Chromatin Structure and its Effects on Transcription

PART V POST-TRANSCRIPTIONAL EVENTS

14. Post-Transcriptional Events I: Splicing
15. Post-Transcriptional Events II: Capping and Polyadenylation
16. Post-Transcriptional Events III: Other Events

PART VI TRANSLATION

17. The Mechanism of Translation I: Initiation
18. The Mechanism of Translation II: Elongation and Termination
19. Ribosomes and Transfer RNA

PART VII DNA REPLICATION, RECOMBINATION, AND TRANSPOSITION

20. DNA Replication I: Basic Mechanism and Enzymology
21. DNA Replication II: Detailed Mechanism

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