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Chapter 17: Microbial And Molecular Genetics |
1. Relevance of Topic
The use of bacteria to mass-produce pharmaceuticals has resulted from the knowledge of recombinant DNA.
Gene mapping and gene cloning is relevant to almost every area of study in biology today, as the technique is used in areas such as phylogenetics, development, and other areas where DNA analysis was never before used.
2. Continuity
The topic of genetic mutants, introduced in the previous chapter is explored in depth in this chapter.
The next chapter, on genetic regulation and engineering, will build on the information presented in this chapter with regard to the basic terminology and mechanisms of gene recombination.
3. Demonstration Activities
Text section 17.1
1. Show Figure 17.1, a genetic map.
Text section 17.2
1. Show Figures 17.2 and 17.3 and discuss mutations.
Text section 17.3
1. Show Figure 17.4 and discuss this plating technique for finding mutants.
2. Figure 17.5 shows temperature-sensitive and host-dependent mutants.
Text section 17.4
1. Show Figures 17.6 an 17.7 in discussing phage crosses and DNA recombination.
Text section 17.5.
1. Show Figure 17.8, a complementation test.
Text section 17.6.
1. Show Figure 17.9 and discuss wild type amino acid sequences.
Text section 17.7.
1. Write out examples of messages and show how they are affected by various mutations.
Text section 17.8.
1. Show Figures 17.10, 17.11, and 17.12, in discussing recombination and plasmids.
Text section 17.10
1. Use computer animation or drawings to illustrate various entities like viruses, plasmids, and phages.
Text section 17.11
1. Show Figure 17.16 in discussing transducers.
Text section 17.13
1. Show Figure 17.17, a mouse cell host system.
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