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Class Activities |
Chapter 18: Gene Regulation And Genetic Engineering 126 |
1. Relevance of Topic
Cloning has become a highly debated topic of interest to all humans, as the cloning of mammals has been demonstrated.
The use of genetics to engineer better foods (vegetables that don't rot as quickly off the vine, livestock feed, even the livestock themselves) is a highly controversial topic.
Separate from the moral issues are medical issues associated with genetic engineering, as certain engineered foods contain genetic material from other organisms (e.g. plants) that may cause allergies or other problems in the uninformed consumer.
Advances in other biological disciplines have been made using the techniques covered in this chapter,and present large potential for solving some of the remaining mysteries in biology.
2. Continuity
This chapter expands on the techniques and mechanisms presented in the last chapter.
The next chapter, on human genetics, becomes more interesting in light of this chapter's material, especially in the area of treatment of genetic disease.
3. Demonstration Activities
Text section 18.1
1. Show Figure 18.1, a block of genes that encode histidine enzymes.
2. Show Figure 18.2, two levels of regulation in bacteria.
Text section 18.2
1. Show Figure 18.3; b-Galactosidase is an inducible enzyme.
2. Figure 18.4 shows two models of gene regulation.
3. Figure 18.6 shows the operon model.
Text section 18.3
1. Figure 18.7 is important in showing three variations on genetic regulation.
Text section 18.4
1. Figure 18.8 can be shown to illustrate a positive mechanism.
Text section 18.5
1. Show Figure 18.9, a general mechanism for repression of biosynthetic operons.
Text section 18.6
1. Figure 18.11, cyclic AMP, shows an alarmone.
Text section 18.7
1. Figure 18.13 shows the basic E. coli lac operon promoter.
Text section 18.8
2. Figure 18.16 illustrates the main idea of this section.
Text section 18.9
1. Figure 18.18 shows steroid hormones; Figure 18.19 shows a model for induction by these hormones.
Text section 18.10
1. Figure 18.20 illustrates the position effect resulting from chromosome inversion.
2. Lampbrush and polytene chromosomes are illustrated in Figures 18.21 and 18.22, respectively.
3. Figures 18.23 and 18.24 show chromosome puffs.
Text section 18.11
1. Figure 18.25 shows an agarose gel.
2. Southern blotting is illustrated in Figure 18.26.
Text section 18.12
1. Figure 18.28 shows a technique for finding genes of interest.
2. The pUC and pET vectors are illustrated in Figure 18.29.
3. Figure 18.30 shows how different bacteria can be distinguished by plating techniques.
Text section 18.13
1. Figure 18.31 shows how the crown gall bacterium works
2. Figure 18.33 shows an example of a genetically engineered tomato, and could also be used during introductory remarks to make the chapter relevant.
Text section 18.14
1. Show figures from recent news stories on the cloning of mammals (e.g. sheep, calves, mice).
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