Chapter 18 Outline


Recombinant DNA and Biotechnology

Gene cloning, biotechnology products, the polymerase chain reaction, DNA fingerprinting, transgenic organisms, gene therapy, and the Human Genome Project are topics covered in this chapter.

Chapter Outline

In this chapter outline, the learning objectives and the selected key terms are given for each major head in the chapter.

Cloning of a Gene (p. 279)

1. Describe the process by which recombinant DNA is prepared and a gene is
cloned.
Selected Key Terms: genetic engineering, recombinant DNA (rDNA), vector,
plasmid, clone, restriction enzyme, DNA ligase

Biotechnology Products Are Many (p. 280)

2. Categorize and list some of the biotechnology products currently on the market.

Replicating Small DNA Segments (p. 281)

3. Describe the polymerase chain reaction; note how and for what purpose the
DNA segments are analyzed.
DNA probe, DNA fingerprinting

Making Transgenic Organisms (p. 283)

4. Describe the services performed by transgenic bacteria.
5. Discuss some of the ecological concerns surrounding transgenic
bacteria.
6. List some types of transgenic plants presently available and those anticipated in
the future.
7. Discuss the use of animals in regard to gene pharming.
transgenic organism

Gene Therapy Is a Reality (p. 285)

8. Outline the current method employed for gene therapy.
9. List illnesses that have been or may be corrected by using gene therapy.
gene therapy

Mapping the Human Chromosome (p. 286)

10. Describe the two purposes of the Human Genome Project.

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