Writing for Botany

Chapter 6 - Numbers


If you can measure that of which you speak, and can express it by a number, you know something of your subject; but if you cannot measure it, your knowledge is meager and unsatisfactory.
William Thomson (Lord Kelvin)

Even in the valley of the shadow of death, two and two do not make six.
Leo Tolstoy

Through and through the world is infested with quantity: to talk sense is to talk quantities. It is no use saying the nation is large-how large? It is no use saying that radium is scarce-how scarce? You cannot evade quantity.
Alfred North Whitehead

Mathematics is both the door and the key to the sciences.
Roger Bacon

Numerical precision is the very soul of science.
Sir D'Arcy Thompson

All science as it grows toward perfection becomes mathematical in its ideas.
Alfred North Whitehead

Botanists try to remove the mystery from the world by using experiments and effective writing to describe and understand plants. Precise language is critical to these descriptions. Since the most precise language is math, it is easy to understand why mathematics is becoming increasingly important in all sciences. For example, consider Gregor Mendel, a botanist and monk who made the most lasting impression on what would become the science of genetics. Mendel's crosses with garden peas were not original; other botanists had already made and studied those crosses for many years. Mendel's genius involved using mathematics to analyze his data. Counting and comparing the different types of offspring enabled Mendel to make a monumental discovery.

Botanists must know how to write effectively about numbers. This requires that botanists understand scientific systems of measurement (i.e., the metric system) as well as how to use statistics to understand populations.

The Metric System

The metric system is a standardized system of measurement used by scientists throughout the world. It is also the measurement system used in everyday life in most countries. Although the metric system is the only measurement system ever acknowledged by Congress, the United States remains "out of step" with the rest of the world by clinging to the antiquated English system of measurements involving pounds, inches, etc.

Metric units commonly used in botany include:
meter (m) The basic unit of length
liter (L) The basic unit of volume
gram (g) The basic unit of mass
degree Celsius (C) The basic unit of temperature
Unlike the English system with which you are already familiar, the metric system is based on units of ten, thus simplifying interconversions. This base-ten system is similar to our monetary system, in which 10 cents equals a dime, 10 dimes equals a dollar, etc. Units of ten in the metric system are indicated by Latin and Greek prefixes placed before the base units. Here are the most common of these prefixes used by botanists:
Prefix Metric Symbol Multiple of Metric Unit
deci d 10-1 = 0.1
centi c 10-2 = 0.01
milli m 10-3 = 0.001
micro µ 10-6 = 0.000001
nano n 10-9 = 0.000000001
pico p 10-12 = 0.000000000001
deka da 101 = 10
hecto h 102 = 100
kilo k 103 = 1000
mega M 106 = 1000000
giga G 109 = 1000000000
tera T 1012 = 1000000000000

Thus, multiply by:

For example, 620 g = 0.620 kg = 620,000 mg = 6200 dg = 62000 cg.

Units of Length

The meter (m) is the basic unit of length.
0.01 to convert cg to g
0.1 to convert dm to m
1000 to convert kg to g
0.000001 to convert to 1
0.1 to convert mm to cm
1 m = 39.4 inches (in) = 1.1 yards (yd) 1 in = 2.54 cm
1 km = 1000 m = 103 m = 0.62 miles 1 ft = 30.5 cm
1 cm = 0.01 m = 10-2 m = 0.39 in = 10 mm 1 yd = 0.91 m
1 nm = 10-9 m = 10-6 mm 1 mi = 1.61 km
470 m = 0.470 km
Units of area are squared (i.e., two-dimensional) units of length:

1 m2 = 1.20 yd2 = 1550 in2 = 1.550 x 103 in2
1 cm2 = 100 mm2
1 hectare = 10000 square meters (m2) = 2.47 acres
Measurements of area and volume can use the same units:
1 m3 = 35.314 ft3 = 1.31 yd3
1 cm3 (cc) = 0.000001 m3 = 0.152 in3

Units of Mass

The gram is the basic unit of mass.
1 g = mass of 1 cm3 of water at 4ºC = 0.035 oz
1 kg = 1000 g = 103 g = 2.2 lb
1 mg = 0.001 g = 10-3 g
Remember that mass is not necessarily synonymous with weight. Mass measures an object's potential to interact with gravity, whereas weight is the force exerted by gravity on an object. A weightless object in outer space has the same mass as it has on earth.

Units of Volume

The liter (L) is the basic unit of volume. Units of volume are cubed (i.e., three-dimensional) units of length.
1 L = 1000 cm3 = 1000 mL 1 tsp = 15 mL
1 L = 2.1 pints = 1.06 qt = 0.26 gal = 1 dm3 1 cup = 0.24 L
1 mL = 0.035 fl oz

Units of Temperature

You are probably most familiar with temperature measured with the Fahrenheit scale, which is based on water freezing at 32ºF and boiling at 212ºF. Celsius temperatures are synonymous with Centigrade temperatures; these scales measure temperature in the metric system. Celsius (C) temperatures are easier to work with than Fahrenheit temperatures since the Celsius scale is based on water freezing at 0ºC and boiling at 100ºC. You can interconvert F and C with the following formula:

5(F) = 9(C) + 160

For example,

40ºC (104ºF) typical for a hot summer day
75ºC (167ºF) hot coffee
-º5C (23ºF) coldest area of freezer
37ºC (98.6ºF) human body temperature

Statistics

Botanists use statistics to reduce a population to a few characteristic numbers such as average height or percent protein. Without statistics, botanists would have to report raw data about each individual. This would be unwieldy as well as frustrating. After all, knowing the protein content of a particular grain of corn (Zea mays) is usually trivial; what we want to know is the protein content of a typical corn grain. To know that, we must study groups of corn grains.

The following is not meant to be a comprehensive description of statistical techniques. Rather, I present only the basic measures you'll need to include in most lab reports. More comprehensive descriptions of statistical techniques are presented elsewhere (Glantz, 1992).
Let's start our discussion with the mean and median.

The mean is the arithmetic average value of the variables.
The median is the middle value of a group of measurements.
The mean is more sensitive to extreme values than is the median. To best appreciate this, consider a group of 14 plants having the following heights: 80 cm, 69 cm, 62 cm, 74 cm, 69 cm, 50 cm, 45 cm, 40 cm, 9 cm, 64 cm, 65 cm, 64 cm, 61 cm, and 67 cm. The mean height is 58.6 cm. However, none of the plants are that height, and most of the plants are taller than 60 cm. Does the mean describe the "typical" plant?

To determine the median, first arrange the measurements in numerical order. Our sample would look like this: 9 cm, 40 cm, 45 cm, 51 cm, 61 cm, 63 cm, 64 cm, 64 cm, 65 cm, 67 cm, 69 cm, 69 cm, 73 cm, 80 cm. The median is between the seventh and eighth measurement; that is, the median is 64 cm. Note that in this example, the mean differs from the median. What causes this difference? How would the mean change if the shortest plant were not in the sample?

The mean of two samples could be the same, yet the samples could differ significantly. For example, consider these samples:
Group 1: 25 cm, 35 cm, 28 cm, 32 cm Mean = 30 cm
Group 2: 10 cm, 15 cm, 20 cm, 75 cm Mean = 30 cm

Thus, reporting only the mean does not give readers a good description of the sample. To better describe the sample, you must provide some measure of the spread and variability of the data. You can do that by reporting the range and standard deviation.

The range is the difference between the largest and smallest values of the sample. For example, the range of Group 1 is 10 (35-25), whereas that of Group 2 is 65 (75-10). Although these measures are informative, they ignore all other data between the extremes.

The standard deviation measures how much each value differs from the mean. It's easy to calculate: to start, calculate the mean, measure the deviation of each sample from the mean, square each deviation, and then sum the deviations. For example, consider a group of trees that are 22 years, 19 years, 21 years, and 18 years old. The mean age is 20 years.
Individual Mean Deviation (Deviation)2
22 20 2 4
19 20 -1 1
21 20 1 1
18 20 -2 4

The sum of the squared deviations is 4 + 1 + 1 + 4 = 10. Divide this number by the number of observations minus one (i.e., 4 - 1 = 3). This produces a value of 10/3 = 3.3 years2, which is the variance (note that the units are years squared). The standard deviation is the square root of the variance: 1.8 years. The standard deviation is usually reported with the mean; for example, "The mean age of the trees was 20 ± 1.8 years."

The standard deviation (SD) is important for understanding the spread of a sample. For many distributions of values for a variable, the mean ± 1 SD encompasses 68% of the observations, whereas the mean ± 2 SD encompasses 95% of the observations. These distributions are the basis for determining what values of some variable are considered normal; those that are not considered normal (in terms of these distributions) may be biologically significant and worthy of more study.

Your future studies in botany will require that you learn more about using statistics to understand plants. For example, you'll need to understand how to do a t test, a chi-square analysis, and an analysis of variance (ANOVA). The specific test that you'll use will depend on the amount and type of your data and the nature of your hypothesis.

Exercises

1. Examine recent issues of Plant Physiology, Scientific American, and Natural History. What is the primary way that authors present data in these periodicals? Plant Physiology:



Scientific American:



Natural History:



2. Scientists are often criticized for their inability to communicate effectively with the public. Is this an accurate criticism? If so, how much of the poor communication results from scientists' inability to present data effectively?







3. Consider these data about the growth of the human population:
Year Population
(millions)
8000 B.C. 5
400 B.C. 86
1 A.D. 133
1650 545
1750 728
1800 906
1850 1130
1900 1610
1950 2400
1960 2998
1970 3659
1980 4551
1990 5300
2000 6500+ (projected)

What would be the best way to present these data to an audience of your peers? Write a short essay describing the importance of these data. Do not repeat the data; rather, discuss what they mean.







4. Re-examine the essay you produced at the end of Chapter 4. If you've not done so already, add some quantitative data to support your ideas. In the space below, describe the source of the data, the importance of the data, and how you would best present those data. Source:



Importance:



Presentation:




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